Review



densitometry fluorchem 8800  (Alpha Innotech)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Alpha Innotech densitometry fluorchem 8800
    Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by <t>densitometry</t> using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.
    Densitometry Fluorchem 8800, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+fluorchem+8800/densitometry+fluorchem+8800/pmc04076351-103-5-8
    Average 90 stars, based on 1 article reviews
    densitometry fluorchem 8800 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Plasma Membrane Ca 2+ -ATPase 4 in Murine Epididymis: Secretion of Splice Variants in the Luminal Fluid and a Role in Sperm Maturation 1 "

    Article Title: Plasma Membrane Ca 2+ -ATPase 4 in Murine Epididymis: Secretion of Splice Variants in the Luminal Fluid and a Role in Sperm Maturation 1

    Journal: Biology of Reproduction

    doi: 10.1095/biolreprod.113.108712

    Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by densitometry using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.
    Figure Legend Snippet: Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by densitometry using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.

    Techniques Used: Western Blot, Expressing, Purification, Control, Software

    Related Articles

    Expressing:

    Article Title: CASK interacts with PMCA4b and JAM-A on the Mouse Sperm Flagellum to Regulate Ca 2+ Homeostasis and Motility
    Article Snippet: .. To quantify the relative expression of CASK, densities of the protein bands were analyzed by densitometry (FluorChem 8800, Alpha Innotech Corp., San Leandro, CA), and the ratio of CASK:HSC70 plotted. .. Co-immunoprecipitation To detect associations between JAM-A and CASK, and CASK and PMCA4 in sperm, coimmunoprecipitation studies were undertaken using sperm protein extracts, containing protease inhibitor CompleteTM (Roche Diagnostics, Indianapolis, IN), prepared in the solubilization buffer, as described above.



    Similar Products

    densitometry fluorchem 8800  (Alpha Innotech)
    90
    Alpha Innotech densitometry fluorchem 8800
    Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by <t>densitometry</t> using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.
    Densitometry Fluorchem 8800, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+fluorchem+8800/densitometry+fluorchem+8800/pmc04076351-103-5-8
    Average 90 stars, based on 1 article reviews
    densitometry fluorchem 8800 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by densitometry using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.

    Journal: Biology of Reproduction

    Article Title: Plasma Membrane Ca 2+ -ATPase 4 in Murine Epididymis: Secretion of Splice Variants in the Luminal Fluid and a Role in Sperm Maturation 1

    doi: 10.1095/biolreprod.113.108712

    Figure Lengend Snippet: Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by densitometry using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.

    Article Snippet: Protein bands were analyzed by densitometry (FluorChem 8800; Alpha Innotech Corp.), and the ratio of PMCA4a to the loading controls was determined and plotted.

    Techniques: Western Blot, Expressing, Purification, Control, Software